1. Cellular biology and virology - Isolation of revertant cells from different cancer cell lines. - Conception and construction of various models for tumor reversion. - Validation through soft agar technique, in vitro transfections and in vivo injections. 2. Differential expression and bio-informatics - mRNA extraction and sequencing. - cDNA replication by PCR technique - Differential gene expression analysis between cancer and revertant cells through differential display technique (MEL), Megasort™ and MPSS™ (LYNX). - Confirmation by Northern Blot, and real time PCR. 3. Function of proteins and Pharmacology - Protein interaction identification through the Yeast Two-Hybrid technique - Elucidation of the functional relevance of protein interactors. - Characterization of the signaling pathways involved in tumor suppression/reversion. - Target validation of potential therapeutic proteins.